The safflower bHLH transcription factor CtbHLH41 negatively regulates SA-induced leaf senescence through interaction with CtCP1

Salicylic acid (SA) plays an important role in regulating leaf senescence. However, the molecular mechanism of senescence safflower ( Carthamus tinctorius ) is still elusive. In this study, we found that bHLH transcription factor (TF) CtbHLH41 significantly delayed and inhibited expression senescence-related genes. order to explore how promotes senescence, carried out yeast two-hybrid screening. by exploring CtCP1 , it was promoted hydrolysis protein, accelerated transcriptional activities salicylic acid-mediated genes CtSAG12 CtSAG29 chlorophyll degradation CtNYC1 CtNYE1 We a negative SA regulator CtANS1 which interacted with regulated its stability, thereby inhibiting -mediated short, our results provide new insight into actively leaves induced SA. • course research on overexpression slowed down included screening two-hybrid, may promote plants. protein also gene interacts delaying caused .